Olga Musharova

Research Scientist
Center of Life Sciences

Supervisor Konstantin Severinov , co-advisor Savitskaya Ekaterina

Entry year to Skoltech – 2013

PhD Thesis Defense:  October 17, 2017, Skoltech

PhD program: Life Sciences

Thesis title: “Investigation of DNA-binding specificity of the Cas1-Cas2 CRISPR adaptation complex in Escherichia coli

Supervisor:  Professor Konstantin Severinov.

Research project – Identification of protein binding sites during the CRISPR-adaptation process.

The CRISPR-Cas adaptive immune systems provide heritable sequence-specific protection against invaders. CRISPR adaptation involves incorporation of invader-derived nucleic acids to the host CRISPR array. The presence of spacer in the CRISPR array against the fully or partially corresponded  foreign DNA strongly increased the efficiency of new spacer incorporation from the same DNA. This process was called primed adaptation. Primed adaptation requires all Cas proteins, but Cas1 and Cas2  are the key participant, so binding activity of Cas-proteins need to be investigated better.

Graduated from -Voronezh State University, 2013, Master of Biology

Traineeship in Laboratory of Microbiology, Wageningen University, the Netherlands (01.2014-06.2014).

2015-2017 UMNIK
(Russian Foundation for Assistance to Small Innovative Enterprises) “Development of the method of complex treatment of bacterial vaginosis”

Olga Musharova “Investigation of DNA-binding specificity of Cas1-Cas2 CRISPR-adaptation complex in Escherichia coli” CRISPR2016, May 23-26, The Weizmann Institute of Science, Rehovot, Israel

Master degree in Biology, graduated with honors

2013- Voronezh State University
Master project: “Investigation of DNA-binding specificity of “zinc finger” domain of BTB-ZF protein family”. Supervisors: Popova T. N.

Determination of PAM requirements for novel Class 2 CRISPR-Cas effectors

  1. Savitskaya EE, Musharova OS, Severinov KV. Diversity of CRISPR-Cas-Mediated Mechanisms of Adaptive Immunity in Prokaryotes and Their Application in Biotechnology. Biochemistry (Mosc). 2016 Jul;81(7):653-61. doi: 10.1134/S0006297916070026
  2. Semenova E, Savitskaya E, Musharova O, Strotskaya A, Vorontsova D, Datsenko KA, Logacheva MD, Severinov K. Highly efficient primed spacer acquisition from targets destroyed by the Escherichia coli type I-E CRISPR-Cas interfering complex. Proc Natl Acad Sci USA.2016 Jul 5;113(27):7626-31. doi: 10.1073/pnas.1602639113. Epub 2016 Jun 20.
  3. Xue C, Seetharam AS, Musharova O, Severinov K, Brouns SJ, Severin AJ, Sashital DG. CRISPR interference and priming varies with individual spacer sequences. Nucleic Acids Res. 2015 Dec 15;43(22):10831-47. doi: 10.1093/nar/gkv1259. Epub 2015 Nov 19.