Supervisor Konstantin Severinov , co-advisor Savitskaya Ekaterina
Entry year to Skoltech – 2013
PhD Thesis Defense: October 17, 2017, Skoltech
PhD program: Life Sciences
Thesis title: “Investigation of DNA-binding specificity of the Cas1-Cas2 CRISPR adaptation complex in Escherichia coli“
Supervisor: Professor Konstantin Severinov.
Research project – Identification of protein binding sites during the CRISPR-adaptation process.
The CRISPR-Cas adaptive immune systems provide heritable sequence-specific protection against invaders. CRISPR adaptation involves incorporation of invader-derived nucleic acids to the host CRISPR array. The presence of spacer in the CRISPR array against the fully or partially corresponded foreign DNA strongly increased the efficiency of new spacer incorporation from the same DNA. This process was called primed adaptation. Primed adaptation requires all Cas proteins, but Cas1 and Cas2 are the key participant, so binding activity of Cas-proteins need to be investigated better.
Graduated from -Voronezh State University, 2013, Master of Biology
Traineeship in Laboratory of Microbiology, Wageningen University, the Netherlands (01.2014-06.2014).
2015-2017 UMNIK (Russian Foundation for Assistance to Small Innovative Enterprises) “Development of the method of complex treatment of bacterial vaginosis”
Olga Musharova “Investigation of DNA-binding specificity of Cas1-Cas2 CRISPR-adaptation complex in Escherichia coli” CRISPR2016, May 23-26, The Weizmann Institute of Science, Rehovot, Israel
Master degree in Biology, graduated with honors
2013- Voronezh State University
Master project: “Investigation of DNA-binding specificity of “zinc finger” domain of BTB-ZF protein family”. Supervisors: Popova T. N.
Determination of PAM requirements for novel Class 2 CRISPR-Cas effectors