olgamusharova

Olga Musharova

Research Scientist
Center of Life Sciences

Supervisor Konstantin Severinov , co-advisor Savitskaya Ekaterina

Entry year to Skoltech – 2013

PhD Thesis Defense:  October 17, 2017, Skoltech

PhD program: Life Sciences

Thesis title: “Investigation of DNA-binding specificity of the Cas1-Cas2 CRISPR adaptation complex in Escherichia coli

Supervisor:  Professor Konstantin Severinov.

Research project – Identification of protein binding sites during the CRISPR-adaptation process.

The CRISPR-Cas adaptive immune systems provide heritable sequence-specific protection against invaders. CRISPR adaptation involves incorporation of invader-derived nucleic acids to the host CRISPR array. The presence of spacer in the CRISPR array against the fully or partially corresponded  foreign DNA strongly increased the efficiency of new spacer incorporation from the same DNA. This process was called primed adaptation. Primed adaptation requires all Cas proteins, but Cas1 and Cas2  are the key participant, so binding activity of Cas-proteins need to be investigated better.

Graduated from -Voronezh State University, 2013, Master of Biology

Traineeship in Laboratory of Microbiology, Wageningen University, the Netherlands (01.2014-06.2014).

Grants:

2018 – 2020 RFBR mol_a  18-34-00048 “Relationship between interference and adaptation during the action of Type I-E CRISPR-Cas system of Escherichia coli”

2015-2017 UMNIK (Russian Foundation for Assistance to Small Innovative Enterprises) “Development of the method of complex treatment of bacterial vaginosis”

Conferences:

Olga Musharova “High – throughput characterization of PAM sequences in Escherichia coli Type I-E CRISPR-Cas system” CRISPR 2018, June 20-23, Vilnius, Lithuania

Olga Musharova “Investigation of DNA-binding specificity of Cas1-Cas2 CRISPR-adaptation complex in Escherichia coli” CRISPR2016, May 23-26, The Weizmann Institute of Science, Rehovot, Israel

Determination of PAM requirements for novel Class 2 CRISPR-Cas effectors

1. Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro. Krivoy A, Rutkauskas M, Kuznedelov K, Musharova O, Rouillon C, Severinov K, Seidel R. Nucleic Acids Res. 2018 May 4;46(8):4087-4098. doi: 10.1093/nar/gky219.
2. Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation. Musharova O, Klimuk E, Datsenko KA, Metlitskaya A, Logacheva M, Semenova E, Severinov K, Savitskaya E. Nucleic Acids Res. 2017 Apr 7;45(6):3297-3307. doi: 10.1093/nar/gkx097.
3. Diversity of CRISPR-Cas-Mediated Mechanisms of Adaptive Immunity in Prokaryotes and Their Application in Biotechnology. Savitskaya EE, Musharova OS, Severinov KV. Biochemistry (Mosc). 2016 Jul;81(7):653-61. doi: 10.1134/S0006297916070026.
4. Highly efficient primed spacer acquisition from targets destroyed by the Escherichia coli type I-E CRISPR-Cas interfering complex. Semenova E, Savitskaya E, Musharova O, Strotskaya A, Vorontsova D, Datsenko KA, Logacheva MD, Severinov K. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):7626-31. doi: 10.1073/pnas.1602639113.
5. CRISPR interference and priming varies with individual spacer sequences. Xue C, Seetharam AS, Musharova O, Severinov K, Brouns SJ, Severin AJ, Sashital DG. Nucleic Acids Res. 2015 Dec 15;43(22):10831-47. doi: 10.1093/nar/gkv1259.

• 2017 PhD in Molecular Biology
Skolkovo Institute of Science and Technology
Supervisors: Severinov KV, Professor, Savitskaya EE, PhD
PhD Project: Investigation of DNA-binding specificity of Cas1-Cas2 CRISPR adaptation
complex in Escherichia coli
• 2013 Master of Biology, graduated with honors
Voronezh State University, Voronezh, Russia
Supervisors: Popova TN,PhD, Zhenilo SV, PhD
MSc Thesis: Investigation of DNA-binding specificity of “zinc finger” domain of BTB-ZF protein family
• 2011 Bachelor of Biology, graduated with honors
Voronezh State University, Voronezh, Russia
Supervisors: Popova TN,PhD, Zhenilo SV, PhD
BSc Thesis: Study of methyl DNA binding transcription factor Kaiso posttranslation modifications

Academic mobility:

Wageningen University, Wageningen, the Netherlands
Laboratory of Microbiology
Supervisor: Stan Brouns, PhD
Project: Investigation of mechanisms protecting cells from autoimmunity during CRISPR interference

01/2014-07/2014