Lidia Putlyaeva

Supervisor: Mikhail Gelfand

Lidia received her diploma at Lomonosov Moscow State University (MSU), Faculty of Biology, in 2011. From 2012 till 2016 Lidia did her PhD thesis at Engelhardt Institute of molecular biology, Moscow. The main part of her work was dedicated to expression process of SLAMF1 gene in human lymphoblasts. She was also involved in the studies of immunopathology-associated polymorphisms of IL2RA and SLAMF1 genes and in research of functional immunomics of the driver oncogenic mutations in well-differentiated thyroid neoplasms.

In Skoltech Lidia will focus on developing of new methodology for assessing epigenetic modifications of histones at the level of individual living cells using fluorescence microscopy. She also takes interest in the evaluation of method of stochastic fluorescence labeling of individual cells and cell lineages

Development of methods of multiparameter fluorescence imaging in biological models. The main goal of the project is to visualize and distinguish various structures and functional processes in cells, tissues and organisms

1. L.V. Putlyaeva, D.E. Demin, K.V. Korneev, A.S. Kasyanov, K.A. Tatosyan, I.V. Kulakovskiy, D.V. Kuprash and A.M. Schwartz. Potential markers of autoimmune diseases, alleles rs115662534(t) and rs548231435(c), disrupt binding of transcription factors STAT1 and EBF1 to regulatory elements of human CD40 gene.

Biochemistry (Mosc). 2018, 83, 12,

DOI: 10.1134/S0006297918120118.

2. A. Uvarova, M. Afanasyeva, L. Putlyaeva, A. Abrosimov, A. Sidorin, A. Schwartz, D. Kuprash, P. Belousov. Autoantigens produced by transformed thyroid cells expressing frequent oncogenic proteins.

FEBS OPEN BIO 8 (Suppl. S1) (2018) p. 465


3. Demin DE, Bogolyubova AV, Zlenko DV, Uvarova AN, Deikin AV, Putlyaeva LV, Belousov PV, Mitkin NA, Korneev KV, Sviryaeva EN, Kulakovskiy IV, Tatosyan KA, Kuprash DV, Schwartz AM. The Novel Short Isoform of Securin Stimulates the Expression of Cyclin D3 and Angiogenesis Factors VEGFA and FGF2, but Does Not Affect the Expression of MYC Transcription Factor.

Mol Biol (Mosk). 2018, 52, 3, 508 – 518

DOI: 10.7868/S002689841803014X.


4. Путляева Л. В., Шварц А. М. Усиление действия селективного ингибитора BRAFV600E на фоне подавления экспрессии гена PTPN11 в клеточной модели рака щитовидной железы. IV Всероссийская Конференция по молекулярной онкологии, 17-19 декабря 2018 г., Москва.

Успехи молекулярной онкологии, приложение к Т.5, №4, с. 52.

5. Putlyaeva LV, Schwartz AM, Klepikova AV, Vorontsov IE, Kulakovskiy IV, Kuprash DV. Single nucleotide polymorphism rs3753381 associated with myasthenia gravis affects SLAMF1 enhancer activity. FEBS Congress, Jerusalem, Israel, 2017.

FEBS Journal. – Vol. 284 (Suppl. 1) – P. 124.


6. Putlyaeva LV, Schwartz AM, Klepikova AV, Vorontsov IE, Kulakovskiy IV, Kuprash DV.

Minor variant of single nucleotide polymorphism rs3753381 affects activity of a SLAMF1 enhancer.

Acta Naturae, (2017), 9, 3(34), 98 – 107.

PMID: 29104781


7. Afanasyeva MA, Putlyaeva LV, Demin DE, Kulakovskiy IV, Vorontsov IE, Fridman MV, Makeev VJ, Kuprash DV, Schwartz AM. The single nucleotide variant rs12722489 determines differential estrogen receptor binding and enhancer properties of an IL2RA intronic region.

PLOS ONE, 2017 Feb 24;12(2):e0172681.

doi: 10.1371/journal.pone.0172681

PMID: 28234966


8. Schwartz AM, Demin DE, Vorontsov IE, Kasyanov AS, Putlyaeva LV, Tatosyan KA, Kulakovskiy IV, Kuprash DV. Multiple single nucleotide polymorphisms in the first intron of the IL2RA gene affect transcription factor binding and enhancer activity.

Gene. 2016 Nov 19. pii: S0378-1119(16)30941-6

doi: 10.1016/j.gene.2016.11.032

PMID: 27876533


9. Schwartz AM, PutlyaevaLV, Covich M, Klepikova AV, Akulich KA, Vorontsov IE, Korneev KV, Dmitriev SE, Polanovsky OL, Sidorenko SP, Kulakovskiy IV, Kuprash DV. Early B-cell factor 1 (EBF1) is critical for transcriptional control of SLAMF1 gene in human B cells.

Biochim Biophys Acta – Gene regulatory mechanisms. 2016 Oct;1859(10):1259-68

doi: 10.1016/j.bbagrm.2016.07.004

PMID: 27424222


10. L.V. Putlyaeva, A.M. Schwartz, D.V. Kuprash. Functional characteristics of single-nucleotide polymorphisms of SLAMF1 gene, associated with autoimmune diseases. Sochi, 2016. V congress of Russian biochemist’s society.

Acta Naturae | (Suppl. 2) – 2016, P. 185.

11. A.M. Schwartz, Putlyaeva L.V., Kulakovskiy I.V., Vorontsov I.E, Demin D.E, Kuprash D.V. Functional characteristics of single-nucleotide polymorphisms of IL2RA gene, associated with autoimmune diseases. Sochi, 2016. V congress of Russian biochemist’s society.

Acta Naturae | (Suppl. 2) – 2016, P. 183.

12. Butvilovskaya VI, Tsybulskaya MV, Tikhonov AA, Talibov VO, Belousov PV, Sazykin AY, Schwartz AM, PutlyaevaLV, Surzhikov SA, Stomakhin AA, Solopova ON, Rubina AY. Preparation of recombinant serpinsв3 and в4 and study of their specific interactions with antibodies using hydrogel-based microarrays.

Mol.Biol(Mosk), 2015 Sep-Oct;49(5):790-799.

doi: 10.7868/S0026898415050043

PMID: 26510597


13. A. M. Shvarts, L. V. Putlyaeva, A. S. Kasianov, M. A. Afanasyeva, M. Covich, K. V. Korneev, I. V. Kulakovskiy, V. J. Makeev, D. V. Kuprash. Functional transcription factor binding sites from the IL2Ra locus that contain SNPs associated with autoimmune pathologies. FEBS Congress, Berlin, 2015.

FEBS Journal. – Vol. 282 (Suppl. 1) – P. 86.


14. LV Putlyaeva, AM Schwartz, KV Korneev, M Covic, L. A. Uroshlev, V. Yu. Makeev, S. E. Dmitriev, D. V. Kuprash. Upstream open reading frames regulate translation of the long isoform of SLAMF1 mRNA that encodes costimulatory receptor CD150.

Biochemistry (Mosk). 2014 Dec; 79(12): 1405-1411.

doi: 10.1134/S0006297914120165

PMID: 25716736


15. L. Putlyaeva, A. M. Schwartz, M. Covic, K. Korneev, D. Kuprash. EBF1 is critical for slamf1 gene expression in human B cells. International Conference “Molecular mechanisms of growth and progression of malignant neoplasms”, Moscow, 2014.

MolBiol (Mosk). – Vol 49 (2015) N 5 p. 762-788 (784).

16. L. Putlyaeva, A. M. Schwartz, K. Korneev, M. Covic, D. Kuprash. Regulation of slamf1 gene encoding costimulatory receptor CD150 in human B cell lines. FEBS EMBO Conference, Paris, 2014.

FEBS Journal. – Vol. 281 (Suppl. 1) – P. 688.


17. Zasedateleva OA, Vasiliskov VA, Surzhikov SA, Sazykin AY, Putlyaeva LV, Schwarz AM, Kuprash DV, Rubina AY, Barsky VE, Zasedatelev AS. UV fluorescence of tryptophan residues effectively measures protein binding to nucleic acid fragments immobilized in gel elements of microarrays.

Biotechnol J. 2014 Aug;9(8):1074-1080.

doi: 10.1002/biot.201300556

PMID: 24924333


2011           defended the specialist’s thesis, “BAX protein and outer mitochondrial membrane permeabilization during the apoptosis”, Lomonosov Moscow State University, Faculty of Biology, Moscow.

2017           defended the PhD thesis, “Regulation of the human SLAMF1 gene expression: the role of the EBF1 transcription factor, single-nucleotide polymorphisms and short   open reading frames”, Engelhardt Institute of Molecular Biology institute of RAS, Moscow.