ilyamazunin

Ilya Mazunin

Senior Research Scientist
Center for Molecular and Cellular Biology

For mtDNA, there are no effective editing systems – that decreases progress in both the investigation of basic mitochondrial biology and the development of methods for gene therapy of hereditary diseases caused by mtDNA mutations. The absence of a reliable system for targeted delivery of biological material to mitochondria is a key problem, which has not been solved in an efficient way yet. In this regard, the development and potentiation of the targeted delivery of therapeutic nucleic acids and proteins to mitochondria will be the cornerstone of our project.

 

  1. Mikhaylova, A.G., Mikhailova, A.A., Ushakova, K., Tretiakov, E.O., Illushchenko, D., Shamansky, V., Lobanova., V.,  Kozenkov, I., Efimenko, B., Yurchenko, A.A., Kozenkova, E., Zdobnov, E., Makeev, V., Yurov, V., Tanaka, M., Gostimskaya, I., Fleischmann, Z., Annis, S., Franco, M., Wasko, K., Denisov, S., Kunz, W., Knorre, D., Mazunin, I., Nikolaev, S., Fellay, J., Reymond, A., Khrapko, K., Gunbin, K., Popadin, K. A mitochondria-specific mutational signature of aging: increased rate of A>G substitutions on a heavy strand (2022) Nucleic Acids Research, 50(18), 10264-10277,  doi: 10.1093/nar/gkac779
  2. Vasilev, R., Gunitseva, N., Shebanova, R., Korzhenkov, A., Vlaskina, A., Evteeva, M., Polushkina, I., Nikitchina, N., Toshchakov, S., Kamenski, P., Patrushev, M., Mazunin, I. Targeted modification of mammalian DNA by a novel Type V Cas12a endonuclease from Ruminococcus bromii (2022) International Journal of Molecular Sciences, Volume 23, Issue 16, 9289, doi: 10.3390/ijms23169289
  3. Tofilo, M., Voskoboeva, E., Timonina, V., Staufner, C., Hoffmann, G., Poschl, J., Schlieben, L., Prokisch, H., Kholodov, D., Ladygin, S., Avdeichik, S., Mazunin, I. A new family with congenital myasthenic syndrome caused by compound heterozygous CHAT mutations (2022) Biomedical Journal of Scientific & Technical Research (BJSTR), doi: 10.26717/BJSTR.2022.44.007122
  4. Kirillova, A., Mazunin, I. Operation “mitochondrial wipeout” – clearing recipient mitochondria DNA during nuclear transfer (2022) Journal of Assisted Reproduction and Genetics, doi: 10.1007/s10815-022-02561-6
  5. Shebanova, R., Nikitchina, N., Shebanov, N., Mekler, V., Kuznedelov, K., Ulashchik, E., Vasilev, R., Sharko, O., Shmanai, V., Tarassov, I., Severinov, K., Entelis, N., Mazunin, I. Efficient target cleavage by Type V Cas12a effector programmed with split CRISPR RNA (2022) Nucleic Acids Research, 50(2), 1162-1173,  doi: 10.1093/nar/gkab1227
  6. Kirillova, A., Smitz, J.E.J., Sukhikh, G., Mazunin, I. The role of mitochondria in oocyte maturation. (2021) Cells, 10(9), 2484. doi: 10.3390/cells10092484
  7. Zakirova, E., Muzyka, V., Mazunin, I., Orishchenko, K. Natural and artificial mechanisms of mitochondrial genome elimination. (2021) Life, 11(2):76. doi: 10.3390/life11020076.
  8. Kenis, V., Melchenko, E., Mazunin, I., Pekkinen, M., Makitie, O. A new family with Epiphyseal chondrodysplasia type Miura (2021) American Journal of Medical Genetics Part A (DOI: 10.1002/ajmg.a.61923) 
  9. Zakirova, E., Vyatkin, Yu, Verechshagina, N., Muzyka, V., Mazunin, I., Orishchenko, K. Study of the effect the introduction of mitochondrial import determinants into the gRNA structure on the activity of the gRNA/SpCas9 complex in vitro (2020). Vavilov Journal of genetics and breeding, 24(5):512-518 (DOI: 10.18699/VJ20.643)
  10. Starikovskaya, E.B., Shalaurova, S.A., Dryomov, S.V., Nazhmidenova, A.M., Volodko, N.V., Bychkov, I.Y., Mazunin, I.O., Sukernik, R.I. Mitochondrial DNA variation of Leber’s Hereditary Optic Neuropathy (LHON) in Western Siberia (2019) Cells, 8, 1574 (DOI: 10.3390/cells8121574)
  11. Gazizova, I.R., Mazunin, I.O.,  Malishevskaya T.N., Kiseleva, O.A., Gadzhiev, A.M., Rindzhibal, Al-M. Mitochondrial DNA as a Factor of Glaucomous Optic Neuropathy’s Development Mechanism (2019) Oftalmologiya; 16(4), pp 479–486 (DOI: 10.18008/1816-5095-2019-4-479-486)
  12. Martemyanov, V., Bykov, R., Demenkova, M., Gninenko, Y., Romancev, S., Bolonin, I., Mazunin, I., Belousova, I., Akhanaev, Y., Pavlushin, S., Krasnoperova, P., Ilinsky, Y. Genetic Evidence of Broad Spreading of Lymantria dispar in the West Siberian Plain (2019) PLOS One (DOI: 10.1371/journal.pone.0220954)
  13. Skuratovskaia, D., Litvinova, L., Zatolokin, P., Vulf, M., Popadin, K., Mazunin, I. From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index (2019) Cells, 8(5) (DOI: 10.3390/cells8050430)
  14. Skuratovskaia, D., Zatolokin, P., Vulf, M., Mazunin, I., Litvinova, L. Interrelation of chemerin and TNF-α with mtDNA copy number in adipose tissues and blood cells in obese patients with and without type 2 diabetes (2019) BMC Medical Genomics (DOI: 10.1186/s12920-019-0485-8)
  15. Litvinova, L., Zatolokin, P., Vulf, M., Mazunin, I., Skuratovskaia, D. The relationship between the mtDNA copy number in insulin-dependent tissues and markers of endothelial dysfunction and inflammation in obese patients (2019) BMC Medical Genomics (DOI: 10.1186/s12920-019- 0486-7)
  16. Bykov, R.A., Yudina, M.A., Gruntenko, N.E., Zakharov, I.K., Voloshina, M.A., Melashchenko, E.S., Danilova, M.V., Mazunin, I.O., Ilinsky, Y.Y. Prevalence and genetic diversity of Wolbachia endosymbiont and mtDNA in Palearctic populations of Drosophila melanogaster (2019) BMC Evolutionary Biology (DOI: 10.1186/s12862-019-1372-9)
  17. Verechshagina, N., Nikitchina, N., Yamada, Y., Harashima, H, Tanaka, M., Orishchenko, K., Mazunin, I. Future of human mitochondrial DNA editing technologies (2019) Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, 30(2), pp. 214-221
  18. Mazunin I. Human mitochondrial genome surgery (2018) Genes & Cells (DOI: 10.23868/201811030)
  19. Verechshagina, N.A., Konstantinov, Y.M., Kamenski, P.A., Mazunin, I.O. Protein and nucleic acid import into mitochondria (2018) Biochemistry (Moscow), 83 (16), pp. 643-661.
  20. Mazunin, I.O., Volodko, N.V. Leber hereditary optic neuropathy (2018) Vestnik Oftalmologii, 134(2), pp. 92-97
  21. Skuratovskaia, D.A., Sofronova, J.K., Zatolokin, P .A., Popadin, K.Y ., Vasilenko, M.A., Litvinova, L.S., Mazunin, I.O. Additional evidence of the link between mtDNA copy number and the body mass index (2018) Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, 29(8), pp. 1240-1244
  22. Skuratovskaia, D.A., Sofronova, J.K., Zatolokin, P .A., Vasilenko, M.A., Litvinova, L.S.,Mazunin, I.O. The association of the mitochondrial DNA OriB variants with metabolic syndrome (2017) Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 63 (6), pp. 533-538.
  23. Sofronova, J.K., Ilinsky, Y.Y., Orishchenko, K.E., Chupakhin, E.G., Lunev, E.A., Mazunin, I.O. Detection of mutations in mitochondrial DNA by droplet digital PCR (2016) Biochemistry (Moscow), 81 (10), pp. 1031-1037.
  24. Orishchenko, K.E., Sofronova, J.K., Chupakhin, E.G., Lunev, E.A., Mazunin, I.O. Delivery Cas9 into mitochondria (2016) Genes & Cells, XI (2), pp. 100-105
  25. Toshchakov, S.V., Korzhenkov, A.A., Samarov, N.I., Mazunin, I.O., Mozhey, O.I., Shmyr, I.S., Derbikova, K.S., Taranov, E.A., Dominova, I.N., Bonch-Osmolovskaya, E.A., Patrushev, M.V., Podosokorskaya, O.A., Kublanov, I.V. Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum (2015) Standards in Genomic Sciences
  26. Mazunin, I.O., Levitskii, S.A., Patrushev, M.V., Kamenski, P.A. Mitochondrial matrix processes (2015) Biochemistry (Moscow), 80 (11), pp. 1418-1428.
  27. Patrushev, M.V., Mazunin, I.O., Vinogradova, E.N., Kamenski, P.A. Mitochondrial fission and fusion (2015) Biochemistry (Moscow), 80 (11), pp. 1457-1464.
  28. Sokhonevich, N.A., Yurova, K.A., Gutsol, A.A., Khaziakhmatova, O.G., Mazunin, I.O., Litvinova, L.S. Effects of Immunoregulatory Cytokines (IL-2, IL-7, and IL-15) on Expression of Gfi1 and U2afll4 Genes in T Cells at Different Stages of Differentiation (2015) Bulletin of Experimental Biology and Medicine, 4 p.
  29. Patrushev, M.V., Kamenski, P.A., Mazunin, I.O. Mutations in mitochondrial DNA and approaches for their correction (2014) Biochemistry (Moscow), 79 (11), pp. 1151-1160.
  30. Mozheĭ, O.I., Zatolokin, P.A., Vasilenko, M.A., Litvinova, L.S., Kirienkova, E.V., Mazunin, I.O. Evaluating the mitochondrial dna copy number in leukocytes and adipocytes from metabolic syndrome patients: pilot study (2014) Molecular Biology, 48 (4), pp. 677-681.
  31. Litvinova, L.S., Kirienkova, E.V., Mazunin, I.O., Vasilenko, M.A., Fattakhov, N.S. Pathogenesis of insulin resistance in metabolic obesity (2014) Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 8 (3), pp. 192-202.
  32. Litvinova, L.S., Mazunin, I.O., Gutsol, A.A., Sokhonevich, N.A., Khaziakhmatova, O.G., Kofanova, K.A. Dose-response effect of steroid hormones on Gfi1 and U2af1l4 gene expression in T lymphocytes at different stages of differentiation (2013) Molecular Biology, 47 (4), pp. 572- 580.
  33. Sukernik, R.I., Volodko, N.V., Mazunin, I.O., Eltsov, N.P., Dryomov, S.V., Starikovskaya, E.B. Mitochondrial genome diversity in the tubalar, even, and ulchi: Contribution to prehistory of native siberians and their affinities to native americans (2012) American Journal of Physical Anthropology, 148 (1), pp. 123-138.
  34. Sukernik, R.I., Volodko, N.V., Mazunin, I.O., Eltsov, N.P., Starikovskaya, E.B. The genetic history of Russian old settlers of polar northeastern Siberia (2010) Russian Journal of Genetics, 46 (11), pp. 1386-1394.
  35. Mazunin, I.O., Volodko, N.V., Starikovskaya, E.B., Sukernik, R.I. Mitochondrial genome and human mitochondrial diseases (2010) Molecular Biology, 44 (5), pp. 665-681.
  36. Mazunin, I.O. Modern concepts of the mitochondrial structure and functions (2010) Russian Journal of Genetics, 46 (9), pp. 1100-1101.
  37. Eltsov, N.P., Volodko, N.V., Starikovskaya, E.B., Mazunin, I.O., Sukernik, R.I. The role of natural selection in the evolution of mitochondrial haplogroups in Northeastern Eurasia (2010) Russian Journal of Genetics, 46 (9), pp. 1105-1107.
  38. Volodko, N.V., Starikovskaya, E.B., Mazunin, I.O., Eltsov, N.P., Naidenko, P.V., Wallace, D.C., Sukernik, R.I. Mitochondrial Genome Diversity in Arctic Siberians, with Particular Reference to the Evolutionary History of Beringia and Pleistocenic Peopling of the Americas (2008) American Journal of Human Genetics, 82 (5), pp. 1084-1100.

Институт цитологии и генетики СО РАН и Институт химической биологии и фундаментальной медицины СО РАН, Новосибирск, Россия

Кандидатская диссертация по биологии, специальность 03.01.07. «молекулярная генетика», декабрь 2010. Тема диссертации: «Анализ изменчивости митохондриальных ДНК тубаларов горного Алтая и эвенов восточной Сибири».

Челябинский государственный университет, Челябинск, Россия

Диплом о высшем биологическом образовании, специализация «генетика», июнь 2006. Тема диплома: «Влияние радиационного фактора на формирование леворукости».