Daria Vorontsova

PhD student

Supervisor – Konstantin Severinov

Entry year to Skoltech – 2013

Date of Thesis Defense: October 24, 2017, 15:00
PhD Program: Life sciences
Thesis: Comparative analysis of the action of eubacterial class 1 CRISPR-Cas systems
Professor Konstantin Severinov
Ekaterina Semenova (Rutgers, The State University of New Jersey)

Research project – The function of subtype I-F CRISPR/Cas systems

CRISPR/Cas systems provide immunity against viruses and plasmids in bacteria and archaea. All of these systems are classified in several types and subtypes with their own structural and functional features. Investigation of each of subtypes provides understanding the mechanisms of action of different CRISPR/Cas systems. It could not only expand our knowledge about life but also give the opportunity to use that information for applied purposes such a production of phage-resistant industrial bacterial strains.

Graduated from St.Petersburg State Polytechnical University, Department of Biophysics


1) Highly efficient primed spacer acquisition from targets destroyed by the Escherichia coli type I-E CRISPR-Cas interfering complex.

Semenova E, Savitskaya E, Musharova O, Strotskaya A, Vorontsova D, Datsenko KA, Logacheva MD, Severinov K.

Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):7626-31. doi: 10.1073/pnas.1602639113

2) Foreign DNA acquisition by the I-F CRISPR-Cas system requires all components of the interference machinery.

Vorontsova D, Datsenko KA, Medvedeva S, Bondy-Denomy J, Savitskaya EE, Pougach K, Logacheva M, Wiedenheft B, Davidson AR, Severinov K, Semenova E.

Nucleic Acids Res. 2015 Dec 15;43(22):10848-60. doi: 10.1093/nar/gkv1261

3) Selective positive modulator of calcium-activated potassium channels exerts beneficial effects in a mouse model of spinocerebellar ataxia type 2.

Kasumu AW, Hougaard C, Rode F, Jacobsen TA, Sabatier JM, Eriksen BL, Strøbæk D, Liang X, Egorova P, Vorontsova D, Christophersen P, Rønn LC, Bezprozvanny I.

Chemistry and Biology, vol 19 (10), 2012, pp. 1340-1353

4) Chronic suppression of inositol 1,4,5-triphosphate receptor-mediated calcium signaling in cerebellar purkinje cells alleviates pathological phenotype in spinocerebellar ataxia 2 mice.

Kasumu AW, Liang X, Egorova P, Vorontsova D, Bezprozvanny I

J Neuroscience Vol.32 (37), 2012, p.1643-12

5) Polyclonal antibodies against human proteasome subunits PSMA3, PSMA5, and PSMB5.

Livinskaya VA, Ivanov VA, Fedorova OA, Vorontsova DN, Barlev NA, Nikiforov AA
Hybridoma (Larchmt). Vol. 31(4), 2012, pp.272-278

M.Sc. Diploma:
Putative RNA polymerases coding by phiKZ bacteriophage
Supervisor: Mikhail Khodorkovsky, PhD.


1) CRISPR-2016, May 23-25, Rehovot, Israel “DNA targeting during interference if III type CRISPR-Cas systems in T. Thermophilus”

2) Information Technologies and Systems” (ITaS)-2016, September 25-30, St. Petersburg, Russia


UMNIK-2015 ” ‘Development of antibiotic-independent screening gene deletions method using the inactivation of genes in thermophilic bacteria Thermus thermophilus’”