valentinafarzan

Valentina Farzan

Junior Research Scientist
Center of Life Sciences

Professor: Timofei Zatsepin

Valentina graduated from Kazan (Volga region) Federal University in 2011. Her diploma research was devoted to the influence of hydration on the receptor properties of beta-cyclodextrins in mass detectors. After graduation, Valentina joined the Laboratory of Analytical studies for Ecological and Environmental Monitoring (Institute of Management of Ecological and Subsurface Problems, Kazan, Russian Federation), where she conducted research in the field of limnology.

In 2013, Valentina joined Central Research Institute of Epidemiology, Moscow, as a Production Associate, where she worked on the oligonucleotide synthesis and led the effort that resulted in the application of novel dyes for qPCR probes. She also made a valuable contribution to the development of fast and robust procedure for oligonucleotide analysis by LC-MS.

During her part-time Junior Researcher appointment in the Laboratory of Tissue-specific Ligands (Chemistry Department, Lomonosov Moscow State University) in 2015, Valentina worked with the conjugation of reactive modified oligonucleotides (DNA, RNA) with small molecules (including GalNAc and metal complexes), purification of oligonucleotides and conjugates by RP HPLC and performed oligonucleotide quality control using LC-MS.

Development of synthesis of modified RNA conjugates with small molecules, including N-galactosamine (asiaglycoprotein receptor in hepatocytes), mannose and folic acid (mannose and folate receptors in macrophages), tocopherol and ligands for PSMA receptor (prostate specific membrane antigene)

Project: “Synthesis of novel modified RNA conjugates for targeted delivery in vivo”

  1. Farzan V.M., Ulashchik E.A., Martynenko-Makaev Y.V., Kvach M.V., Aparin I.O., Brylev V.A., Prikazchikova T.A., Maklakova S.Y., Majouga A.G., Ustinov A.V., Shipulin G.A., Shmanai V.V., Korshun V.A., Zatsepin T.S. Automated Solid-Phase Click Synthesis of Oligonucleotide Conjugates: From Small Molecules to Diverse N-Acetylgalactosamine Clusters. Bioconjugate Chemistry, 28(10) (2017) 2599-2607
  2. Valentina M. Farzan, Mikhail L. Markelov, Alexander Yu. Skoblov, German A. Shipulin and Timofei S. Zatsepin.  Specificity of SNP detection with molecular beacons is improved by stem and loop separation with spacers. Analyst142 (2017) 945-950
  3. Farzan V.M., Aparin I.O., Veselova O.A., Podkolzin A.T., Shipulin G.A., Korshun V.A., Zatsepin T.S. Cy5/BHQ dye–quencher pairs in fluorogenic qPCR probes: effects of charge and hydrophobicity. Analytical Methods  8(29) (2016) 5826-5831.
  4. Ilya O. Aparin, Valentina M. Farzan, Olga A. Veselova, Alexey A. Chistov, Alexander T. Podkolzin, Alexey V. Ustinov, German A. Shipulin, Andrey A. Formanovsky, Vladimir A. Korshun and Timofei S. Zatsepin. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst 141 (2016) 1331-1338.
  5. Aleksander Yu. Skoblov, Maxim V. Vichuzhanin, Valentina M. Farzan, Olga A. Veselova, Tatiana A. Konovalova, Alexander T. Podkolzin, German A. Shipulin, Timofei S. Zatsepin. Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes. Bioorganic & Medicinal Chemistry 23 (2015) 6749–6756.

2011 M. Sc. in Chemistry, Department of Chemistry (Physical Chemistry), Kazan (Volga region) Federal University, Kazan, Russia.   M. Sc. thesis: “Influense of hydration on the receptor properties of beta-cyclodextrins in mass detectors”. Supervisor Dr. V.V.Gorbachuk.