mariasokolova

Maria Sokolova

Research Scientist

PhD Thesis Defense: June 15, 2018, Skoltech

PhD program: Life Sciences

Thesis title: “Functional and structural analysis of a non-canonical multisubunit RNA polymerase encoded by giant bacteriophage AR9”

Supervisor: Professor Konstantin Severinov

 

  • Structural and functional characterization of non-canonical viral RNA polymerases
  • Development, approbation, and delivery of Master and PhD level practical laboratory courses on “Molecular machines” and a research seminar on the same topic

A non-canonical multisubunit RNA polymerase encoded by the AR9 phage recognizes the template strand of its uracil-containing promoters. Sokolova M, Borukhov S, Lavysh D, Artamonova T, Khodorkovskii M, Severinov K. Nucleic Acids Res. 2017 Jun 2;45(10):5958-5967. doi: 10.1093/nar/gkx264.

 Transcription Profiling of Bacillus subtilis Cells Infected with AR9, a Giant Phage Encoding Two Multisubunit RNA Polymerases. Lavysh D, Sokolova M, Slashcheva M, Forstner KU, Severinov K.В MBio. 2017 Feb 14;8(1). pii: e02041-16. doi: 10.1128/mBio.02041-16.

The genome of AR9, a giant transducing Bacillus phage encoding two multisubunit RNA polymerases. Lavysh D, Sokolova M, Minakhin L, Yakunina M, Artamonova T, Kozyavkin S, Makarova KS, Koonin EV, Severinov K. Virology.В 2016 May 26;495:185-196. doi: 10.1016/j.virol.2016.04.030.

Grants:

RFBR mol_a  14-04-32389

Investigation of TIP49 eukaryotic proteins

2014-2015

Maria Sokolova participated in  Science and Technology in Society Forum (STS forum)

Maria Sokolova organized and led Skoltech summer school

 

Conference participation:

Transcription Strategy of a Giant Bacteriophage AR9 and Characterization of Its Non-Canonical Multisubunit RNA Polymerase.

Mechanism and Regulation of Prokaryotic Transcription, Federation of American Societies for Experimental Biology (FASEB), Saxtons River, VT, USA, 25-30 June 2017

Transcription Strategy of a Giant Bacteriophage AR9 and Characterization of Its Non-Canonical Multisubunit RNA Polymerase.

22nd Structural Biology Symposium, Sealy Center for Structural Biology and Molecular Biophysics Symposium, The University of Texas Medical Branch, TX, USA, 6 May 2017

 A non-canonical multisubunit RNA polymerase encoded by the AR9 phage recognizes the template strand of its uracil-containing promoters.

29th  RNA Polymerase Workshop, Newcastle University, UK,  6-7 April 2017

Functional analysis of AR9 bacteriophage and characterization of its non-canonical multisubunit RNA polymerase.

Bacteriophages: Theoretical and Practical Aspects of Their Application in Medicine, Veterinary and Food, Moscow, Russia, 13-15 October 2016

2nd place for the Poster Session

 Functional analysis of AR9 bacteriophage and characterization of its non-canonical multisubunit RNA polymerase.

Interdisciplinary School and Conference “Information Technologies and Systems” ITAS, Saint-Petersburg, Russia, 25-30 September 2016

 Unusual RNA polymerases encoded by the PBS1 phage.

76th Harden Conference: Total Transcription, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, 1-5 September 2014

 

Internships:

February 2017 – December 2017, Studying of the AR9 RNA polymerase structure at Petr Leiman`s laboratory at The University of Texas Medical Branch, TX, USA

https://scsb.utmb.edu/faculty/Leiman.asp