ilyaosterman

Ilya Osterman

Principal Research Scientist
Center for Molecular and Cellular Biology

Professor: Olga Dontsova

Dr Ilya Osterman is a principal researcher at  Skoltech Center of Life Sciences and researcher at the Department of Chemistry, Lomonosov Moscow State University.  Ilya finished MSU in 2009 and started his PhD project in the field of RNA modifications. In 2012 he received his PhD degree and became a researcher at the Department of Chemistry, in 2018 he  obtained his habilitation in chemistry and became principal researcher at  Skoltech Center of Life Sciences . Dr. Osterman is a winner of many prestigious prizes for young scientists, the European Academy prize, the medal of Russian Academy of Science and prizes of Russian and Moscow Government among them.​

The main subject of Ilya work refers to RNA-protein complexes in bacteria. During PhD project, he systematically studied how structural variations in 5’-UTR and other features of bacterial genes including Shine-Dalgarno sequence (SD), distinct start codons, A/U-rich regions, mRNA secondary structure, codon usage, organization of genes in the operons affect the efficiency of translation.

Ilya created screening approaches to identify new antibiotics and recently characterized a number of translation inhibitors with novel chemical scaffolds. One of them,  Amicoumacin A was characterized in detail, including structure determination (in collaboration by Tom Steitz, the Nobel prize winner for structure of ribosome). These studies vividly demonstrated the fundamental and drug discovery impacts of the new methodology.

Recently, several new translational inhibitors were characterized in details by means of microbiological and structural methods.

Also, Ilya  work with eukaryotic cells, he is trying to find the targets for the putative methyltransferases and to investigate their function, by means of genome editing with CRISP-Cas9 system in cell lines and in mice.

​At Skoltech, Ilya works in the area of eukaryotic genome editing and continues  screening of new translation inhibitors

 

 

  1. Osterman IA, Wieland M, Maviza TP, Lashkevich KA, Lukianov DA, Komarova ES, Zakalyukina YV, Buschauer R, Shiriaev DI, Leyn SA, Zlamal JE, Biryukov MV, Skvortsov DA, Tashlitsky VN, Polshakov VI, Cheng J, Polikanov YS, Bogdanov AA, Osterman AL, Dmitriev SE, Beckmann R, Dontsova OA, Wilson DN, Sergiev PV. Tetracenomycin X inhibits translation by binding within the ribosomal exit tunnel. Nat Chem Biol. 2020
  2. Osterman IA, Chervontseva ZS, Evfratov SA, Sorokina AV, Rodin VA, Rubtsova MP, Komarova ES, Zatsepin TS, Kabilov MR, Bogdanov AA, Gelfand MS, Dontsova OA, Sergiev PV. Translation at first sight: the influence of leading codons. Nucleic Acids Res. 2020 9;48(12):6931-6942
  3. Pichkur EB, Paleskava A, Tereshchenkov AG, Kasatsky P, Komarova ES, Shiriaev DI, Bogdanov AA, Dontsova OA, Osterman IA, Sergiev PV, Polikanov YS, Myasnikov AG, Konevega AL. Insights into the improved macrolide inhibitory activity from the high-resolution cryo-EM structure of dirithromycin bound to the E. coli 70S ribosome. RNA.2020
  4. Ovchinnikov SV, Bikmetov D, Livenskyi A, Serebryakova M, Wilcox B, Mangano K, Shiriaev DI, Osterman IA, Sergiev PV, Borukhov S, Vazquez-Laslop N, Mankin AS, Severinov K, Dubiley S. Mechanism of translation inhibition by type II GNAT toxin AtaT2. Nucleic Acids Res. 2020
  5. Y. Travin, Z. L. Watson, M. Metelev, F. R. Ward, I. A. Osterman, I. M. Khven, N. F. Khabibullina, M. Serebryakova, P. Mergaert, Y. S. Polikanov, J. H. D. Cate and K. Severinov, “Cryo-EM structure of ribosome-bound azole-modified peptide phazolicin rationalizes its species-specific mode of bacterial translation inhibition,” Nat. Comms., 2019
  6. Ivanenkov YA, Zhavoronkov A, Yamidanov RS, Osterman IA, Sergiev PV, Aladinskiy VA, Aladinskaya AV, Terentiev VA, Veselov MS, Ayginin AA, Kartsev VG, Skvortsov DA, Chemeris AV, Baimiev AK, Sofronova AA, Malyshev AS, Filkov GI, Bezrukov DS, Zagribelnyy BA, Putin EO, Puchinina MM, Dontsova OA. Identification of Novel Antibacterials Using Machine Learning Techniques. Front Pharmacol. 2019
  7. Khabibullina NF, Tereshchenkov AG, Komarova ES, Syroegin EA, Shiriaev DI, Paleskava A, Kartsev VG, Bogdanov AA, Konevega AL, Dontsova OA, Sergiev PV, Osterman IA, Polikanov YS. Structure of Dirithromycin Bound to the Bacterial Ribosome Suggests New Ways for Rational Improvement of Macrolides. Antimicrob Agents Chemother. 2019 May 24;63(6).
  8. Zakalyukina YV, Birykov MV, Lukianov DA, Shiriaev DI, Komarova ES, Skvortsov DA, Kostyukevich Y, Tashlitsky VN, Polshakov VI, Nikolaev E, Sergiev PV, Osterman IA. Nybomycin-producing Streptomyces isolated from carpenter ant Camponotus vagus. 2019
  9. Tevyashova AN, Korolev AM, Mirchink EP, Isakova EB, Osterman IA. Synthesis and evaluation of biological activity of benzoxaborole derivatives of azithromycin. J Antibiot (Tokyo).
  10. Terekhov SS, Smirnov IV, Malakhova MV, Samoilov AE, Manolov AI, Nazarov AS, Danilov DV, Dubiley SA, Osterman IA, Rubtsova MP, Kostryukova ES, Ziganshin RH, Kornienko MA, Vanyushkina AA, Bukato ON, Ilina EN, Vlasov VV, Severinov KV, Gabibov AG, Altman S. Ultrahigh-throughput functional profiling of microbiota communities. 2018. Proc Natl Acad Sci U S A
  11. Terekhov SS, Osterman IA, Smirnov IV. High-Throughput Screening of Biodiversity for Antibiotic Discovery. Acta Naturae.
  12. Jiang ZK, Tuo L, Huang DL, Osterman IA, Tyurin AP, Liu SW, Lukyanov DA, Sergiev PV, Dontsova OA, Korshun VA, Li FN, Sun CH. Diversity, Novelty, and Antimicrobial Activity of Endophytic Actinobacteria From Mangrove Plants in Beilun Estuary National Nature Reserve of Guangxi, China. Front Microbiol. 2018
  13. Wilcox B*, Osterman I*, Serebryakova M, Lukyanov D, Komarova E, Gollan B, Morozova N, Wolf YI, Makarova KS, Helaine S, Sergiev P, Dubiley S, Borukhov S, Severinov K. Escherichia coli ItaT is a type II toxin that inhibits translation by acetylating isoleucyl-tRNAIle. Nucleic Acids Res. 2018 * Authors contributed equally to this work
  14. Travin DY, Metelev M, Serebryakova M, Komarova ES, Osterman IA, Ghilarov D, Severinov K. Biosynthesis of Translation Inhibitor Klebsazolicin Proceeds through Heterocyclization and N-Terminal Amidine Formation Catalyzed by a Single YcaO Enzyme. J Am Chem Soc. 2018
  15. Tereshchenkov AG, Dobosz-Bartoszek M, Osterman IA, Marks J, Sergeeva VA, Kasatsky P, Komarova ES, Stavrianidi AN, Rodin IA, Konevega AL, Sergiev PV, Sumbatyan NV, Mankin AS, Bogdanov AA, Polikanov YS. Binding and Action of Amino Acid Analogs of Chloramphenicol upon the Bacterial Ribosome. J Mol Biol. 2018
  16. Metelev, M.*, Osterman, I.A.*, Ghilarov, D., Khabibullina, N.F., Yakimov, A., Shabalin, K., Utkina, I., Travin, D.Y., Komarova, E.S., Serebryakova, M., Artamonova, T., Khodorkovskii, M., Konevega, A.L., Sergiev, P.V., Severinov, K., Polikanov, Y.S. Klebsazolicin inhibits 70S ribosome by obstructing the peptide exit tunnel (2017) Nature Chemical Biology  Oct;13(10):1129-1136 * Authors contributed equally to this work
  17. Osterman IA, Khabibullina NF, Komarova ES, Kasatsky P, Kartsev VG, Bogdanov AA, Dontsova OA, Konevega AL, Sergiev PV, Polikanov YS. Madumycin II inhibits peptide bond formation by forcing the peptidyl transferase center into an inactive state (2017). Nucleic Acids Res
  18. Nazarov PA, Osterman IA, Tokarchuk AV, Karakozova MV, Korshunova GA, Lyamzaev KG, Skulachev MV, Kotova EA, Skulachev VP, Antonenko YN. Mitochondria-targeted antioxidants as highly effective antibiotics. 2017 Scientific Reports. May 3;7(1):1394.
  19. Komarova (Andreyanova), E.S., Osterman, I.A., Pletnev, P.I., Ivanenkov, Y.A., Majouga, A.G., Bogdanov, A.A., Sergiev, P.V. 2-Guanidino-quinazolines as a novel class of translation inhibitors (2017) Biochimie, 133, pp. 45-55.
  20. Evfratov SA*, Osterman IA*, Komarova ES*, Pogorelskaya AM, Rubtsova MP, Zatsepin TS, Semashko TA, Kostryukova ES, Mironov AA, Burnaev E, Krymova E, Gelfand MS, Govorun VM, Bogdanov AA, Sergiev PV, Dontsova OA. Application of sorting and next generation sequencing to study 5′-UTR influence on translation efficiency in Escherichia coli(2017). Nucleic Acids Res. Apr 7;45(6):3487-3502. * Authors contributed equally to this work
  21. Osterman, I.A., Komarova, E.S., Shiryaev, D.I., Korniltsev, I.A., Khven, I.M., Lukyanov, D.A., Tashlitsky, V.N., Serebryakova, M.V., Efremenkova, O.V., Ivanenkov, Y.A., Bogdanov, A.A., Sergiev, P.V., Dontsova, O.A. Sorting out antibiotics’ mechanisms of action: A double fluorescent protein reporter for high-throughput screening of ribosome and DNA biosynthesis inhibitors (2016) Antimicrobial Agents and Chemotherapy, 60 (12), pp. 7481-7489.
  22. Sergiev, P.V., Osterman, I.A., Golovina, A.Y., Andreyanova, E.S., Laptev, I.G., Pletnev, P.I., Evfratov, S.A., Marusich, E.I., Leonov, S.V., Ivanenkov, Y.A., Bogdanov, A.A., Dontsova, O.A. Application of reporter strains for screening of new antibiotics (2016) Biochemistry (Moscow)
  23. Prokhorova, I.V., Akulich, K.A., Makeeva, D.S., Osterman, I.A., Skvortsov, D.A., Sergiev, P.V., Dontsova, O.A., Yusupova, G., Yusupov, M.M., Dmitriev, S.E. Amicoumacin A induces cancer cell death by targeting the eukaryotic ribosome (2016) Scientific Reports, 6, art. no. 27720, .
  24. Sergiev, P.V., Golovina, A.Y., Osterman, I.A., Nesterchuk, M.V., Sergeeva, O.V., Chugunova, A.A., Evfratov, S.A., Andreianova, E.S., Pletnev, P.I., Laptev, I.G., Petriukov, K.S., Navalayeu, T.I., Koteliansky, V.E., Bogdanov, A.A., Dontsova, O.A. N6-Methylated Adenosine in RNA: From Bacteria to Humans (2016) Journal of Molecular Biology, 428 (10), pp. 2134-2145.
  25. Beloglazkina, E.K., Manzheliy, E.A., Moiseeva, A.A., Maloshitskaya, O.A., Zyk, N.V., Skvortsov, D.A., Osterman, I.A., Sergiev, P.V., Dontsova, O.A., Ivanenkov, Y.A., Veselov, M.S., Majouga, A.G. Synthesis, characterisation, cytotoxicity and antibacterial activity of ruthenium(II) and rhodium(III) complexes with sulfur-containing terpyridines (2016) Polyhedron, 107, pp. 27-37.
  26. Sergiev, P.V., Osterman, I.A., Golovina, A.Y., Andreyanova, E.S., Laptev, I.G., Pletnev, F.I., Evfratov, S.A., Marusich, E.I., Veselov, M.S., Leonov, S.V., Ivanenkov, Y.A., Bogdanov, A.A., Dontsova, O.A. High throughput screening platform for new inhibitors of protein biosynthesis (2016) Moscow University Chemistry Bulletin, 71 (1), pp. 65-67.
  27. Sergiev, P.V., Osterman, I.A., Golovina, A.Ya., Laptev, I.G., Pletnev, P.I., Evfratov, S.A., Marusich, E.I., Leonov, S.V., Ivanenkov, Ya.A., Bogdanov, A.A., Dontsova, O.A. Application of reporter strains for new antibiotic screening (2016) Biomeditsinskaya Khimiya, 62 (2), pp. 117-123.
  28. Osterman, I.A., Dikhtyar, Yu.Yu., Bogdanov, A.A., Dontsova, O.A., Sergiev, P.V. Regulation of flagellar gene expression in Bacteria (2015) Biochemistry (Moscow), 80 (11), pp. 1447-1456.
  29. Pletnev, P., Osterman, I., Sergiev, P., Bogdanov, A., Dontsova, O. Survival guide: Escherichia coli in the stationary phase (2015) Acta Naturae, 7 (4), pp. 22-33.
  30. Veselov, M.S., Sergiev, P.V., Osterman, I.A., Skvortsov, D.A., Golovina, A.Ya., Andreyanova, E.S., Laptev, I.G., Pletnev, P.I., Evfratov, S.A., Marusich, E.I., Leonov, S.V., Ivanenkov, Y.A., Bogdanov, A.A., Dontsova, O.A. Common features of antibacterial compounds: An analysis of 104 compounds library (2015) Biomeditsinskaya Khimiya, 61 (6), pp. 785-790.
  31. Osterman, I.A., Evfratov, S.A., Dzama, M.M., Pletnev, P.I., Kovalchuk, S.I., Butenko, I.O., Pobeguts, O.V., Golovina, A.Y., Govorun, V.M., Bogdanov, A.A., Sergiev, P.V., Dontsova, O.A. A bacterial homolog YciH of eukaryotic translation initiation factor eIF1 regulates stress-related gene expression and is unlikely to be involved in translation initiation fidelity (2015) RNA Biology, 12 (9), pp. 966-971.
  32. Zukher, I., Novikova, M., Tikhonov, A., Nesterchuk, M.V., Osterman, I.A., Djordjevic, M., Sergiev, P.V., Sharma, C.M., Severinov, K. Ribosome-controlled transcription termination is essential for the production of antibiotic microcin C (2014) Nucleic Acids Research, 42 (19), pp. 11891-11902.
  33. Polikanov, Y.S.*, Osterman, I.A.*, Szal, T., Tashlitsky, V.N., Serebryakova, M.V., Kusochek, P., Bulkley, D., Malanicheva, I.A., Efimenko, T.A., Efremenkova, O.V., Konevega, A.L., Shaw, K.J., Bogdanov, A.A., Rodnina, M.V., Dontsova, O.A., Mankin, A.S., Steitz, T.A., Sergiev, P.V. Amicoumacin A Inhibits Translation by Stabilizing mRNA Interaction with the Ribosome (2014) Molecular Cell, 56 (4), pp. 531-540. * Authors contributed equally to this work
  34. Prokhorova, I.V.*, Osterman, I.A.*, Burakovsky, D.E., Serebryakova, M.V., Galyamina, M.A., Pobeguts, O.V., Altukhov, I., Kovalchuk, S., Alexeev, D.G., Govorun, V.M., Bogdanov, A.A., Sergiev, P.V., Dontsova, O.A.Modified nucleotides m 2 G966/m 5 C967 of Escherichia coli 16S rRNA are required for attenuation of tryptophan operon (2013) Scientific Reports * Authors contributed equally to this work
  35. Osterman, I.A., Evfratov, S.A., Sergiev, P.V., Dontsova, O.A. Comparison of mRNA features affecting translation initiation and reinitiation (2013) Nucleic Acids Research, 41 (1), pp. 474-486.
  36. Shatskaya, G.S., Drutsa, V.L., Koroleva, O.N., Osterman, I.A., Dmitrieva, T.M. Investigation of activity of recombinant mengovirus RNA-dependent RNA polymerase and its mutants (2013) Biochemistry (Moscow), 78 (1), pp. 96-101.
  37. Osterman, I.A., Ustinov, A.V., Evdokimov, D.V., Korshun, V.A., Sergiev, P.V., Serebryakova, M.V., Demina, I.A., Galyamina, M.A., Govorun, V.M., Dontsova, O.A. A nascent proteome study combining click chemistry with 2DE (2013) Proteomics, 13 (1), pp. 17-21.
  38. Golovina, A.Y., Dzama, M.M., Osterman, I.A., Sergiev, P.V., Serebryakova, M.V., Bogdanov, A.A., Dontsova, O.A. The last rRNA methyltransferase of E. coli revealed: The yhiR gene encodes adenine-N6 methyltransferase specific for modification of A2030 of 23S ribosomal RNA (2012) RNA, 18 (9), pp. 1725-1734.
  39. Sergiev, P.V., Golovina, A.Y., Sergeeva, O.V., Osterman, I.A., Nesterchuk, M.V., Bogdanov, A.A., Dontsova, O.A. How much can we learn about the function of bacterial rRNA modification by mining large-scale experimental datasets? (2012) Nucleic Acids Research, 40 (12), pp. 5694-5705.
  40. Osterman, I.A., Prokhorova, I.V., Sysoev, V.O., Boykova, Y.V., Efremenkova, O.V., Svetlov, M.S., Kolb, V.A., Bogdanov, A.A., Sergiev, P.V., Dontsova, O.A. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors(2012) Antimicrobial Agents and Chemotherapy, 56 (4), pp. 1774-1783.
  41. Osterman, I.A., Sergiev, P.V., Tsvetkov, P.O., Makarov, A.A., Bogdanov, A.A., Dontsova, O.A. Methylated 23S rRNA nucleotide m2G1835 of Escherichia coli ribosome facilitates subunit association (2011) Biochimie, 93 (4), pp. 725-729.
  42. Sergiev, P.V., Osterman, I.A., Prokhorova, I.V., Nesterchuk, M.V., Sergeeva, O.V., Golovina, A.I., Demina, I.A., Galiamina, M.A., Serebriakova, M.V., Dontsova, O.A. [Systems biology approach to the functional role of enzymatic modification of bacterial ribosome]. (2011) Bioorganicheskaia khimiia, 37 (1), pp. 81-90.
  43. Sergiev, P.V., Osterman, I.A., Prokhorova, I.V., Nesterchuk, M.V., Sergeeva, O.V., Golovina, A.Ya., Demina, I.A., Galyamina, M.A., Serebryakova, M.V., Dontsova, O.A. The study of the functional role of enzymatic modifications in bacterial ribosomes by the system biology approach (2011) Russian Journal of Bioorganic Chemistry, 37 (1), pp. 71-79.

2004 – 2009                Specialist, Lomonosov MSU, Chemistry Dept, Moscow, Russia

2009 – 2012                PhD, Lomonosov MSU, Chemistry Dept, Moscow, Russia

Screening and study of new antibiotics – translation inhibitors and SOS-response inducers

Genome editing of antibiotic producers.

Bacterial translation and ribosome.

Study of the functional role of the eukaryoyic methyltransferasas by means of genome knock-out in mice or cell lines with CRISPR-Cas9 systems.

2010 –  winner of the contest of innovative projects “I5”.

2011 – RSBMB Russian Society of Biochemistry and Molecular Biology

2013 – winner of the contest “Impudent Young Promising”

2013 – winner of the European Academy prize for young scientist

2014 – winner of the grant for young biologist from Dynastia fund

2012 and 2015 – winner presidential scholarship for young scientist

2015 – winner of the contest for young scientists of the Lomonosov Moscow State University

2015 – winner of the medal of Russian academy of science for young scientists

2016 – winner of the prize of Russian government for young scientists

2019  – winner of the prize of Moscow government for young scientists