Iana Fedorova

Research advisor: Konstantin Severinov

Program entry year: 2014

Graduated from Peter the Great St.Petersburg Polytechnic Universityin 2013

Research project: Studying of CRISPR Cas proteins and using them for genome-editing

Research interests:  Bacterial CRISPR Cas enzymes provide a specific and at the same time flexible link between nucleic acids and proteins: they can bind DNA/RNA sequences through compliment pairing of the target with crRNA. This ability of specific nucleic acids recognition and binding is used for development of numerous molecular biology tools: genome editing instruments, transcription regulation systems, elements of genetic circuits, detection systems and others. My research interest is to study diverse Cas-enzymes and make different tools based on their properties.

 Academic mobility:

University: Broad Institute, MIT https://zlab.bio

Laboratory: Feng Zhang laboratory

Dates: November 2015-November 2016

Publications:

• Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017. Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F.
• Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA. Cell. 2016.Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O.
• BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks. Nat Commun. 2017. Yan WX, Mirzazadeh R, Garnerone S, Scott D, Schneider MW, Kallas T, Custodio J, Wernersson E, Li Y, Gao L, Federova Y, Zetsche B, Zhang F, Bienko M, Crosetto N.

Conference presentations:

2017, Skoltech & MIT Conference: “Shaping the Future: Big Data, Biomedicine and Frontier Technologies” (poster)

2018, “Biotech Club”, Moscow, Russia (oral presentation)

2019, “Future Biotech”, Saint Petersburg, Russia (oral presentation)

2019, “CRISPR 2019” Quebec, Canada (poster)