evgenyklimuk

Evgeny Klimuk

Research Scientist
Center of Life Sciences

Professor: Konstantin Severinov

 

 

 

Analysis of bacteriophage interactions with bacterial hosts and of phage DNA packaging in virions

Oliver W Bayfield, Evgeny Klimuk, Dennis C Winkler, Emma L Hesketh, Maria Chechik, Naiqian Cheng, Eric C Dykeman, Leonid Minakhin, Neil A Ranson, Konstantin Severinov, Alasdair C Steven, Alfred A. Antson: Cryo-EM structure and in vitro DNA packaging of a thermophilic virus with supersized T=7 capsids. Proceedings of the National Academy of Sciences 02/2019; 116(9)., DOI:10.1073/pnas.1813204116

Evgeny Klimuk, Ekaterina Bogdanova, Max Nagornykh, Andjela Rodic, Marko Djordjevic, Sofia Medvedeva, Olga Pavlova, Konstantin Severinov: Controller protein of restriction-modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock. Nucleic Acids Research 10/2018; 46(20)., DOI:10.1093/nar/gky880

Anastasia V Popova, Daria G Lavysh, Evgeniy I Klimuk, Mikhail V Edelstein, Alexander G Bogun, Mikhail M Shneider, Artemiy E Goncharov, Sergey V Leonov, Konstantin V Severinov: Novel Fri1‐like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12— Characterization, Comparative Genomic Analysis, and Host‐Recognition Strategy. Viruses 07/2017; 2017(9)., DOI:10.3390/v9070188

Olga Musharova, Evgeny Klimuk, Kirill A. Datsenko, Anastasia Metlitskaya, Maria Logacheva, Ekaterina Semenova, Konstantin Severinov, Ekaterina Savitskaya: Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation. Nucleic Acids Research 02/2017; 45(6)., DOI:10.1093/nar/gkx097

Rui-Gang Xu, Huw Thomas Jenkins, Maria Chechik, Elena V Blagova, Anna Lopatina, Evgeny Klimuk, Leonid Minakhin, Konstantin Severinov, Sandra Joy Muriatu Greive, Alfred Aksel Antson: Viral genome packaging terminase cleaves DNA using the canonical RuvC-like two-metal catalysis mechanism. Nucleic Acids Research 01/2017; 45(6)., DOI:10.1093/nar/gkw1354

Daria Esyunina, Evgeny Klimuk, Konstantin Severinov, Andrey Kulbachinskiy: Distinct pathways of RNA polymerase regulation by a phage-encoded factor. Proceedings of the National Academy of Sciences 02/2015; 112(7)., DOI:10.1073/pnas.1416330112

Pieter-Jan Ceyssens, Leonid Minakhin, An Van den Bossche, Maria Yakunina, Evgeny Klimuk, Bob Blasdel, Jeroen De Smet, Jean-Paul Noben, Udo Bläsi, Konstantin Severinov, Rob Lavigne: Development of Giant Bacteriophage KZ Is Independent of the Host Transcription Apparatus. Journal of Virology 09/2014; 88(18):10501-10., DOI:10.1128/JVI.01347-14

Evgeny Klimuk, Natalia Akulenko, Kira S Makarova, Pieter-Jan Ceyssens, Ivan Volchenkov, Rob Lavigne, Konstantin Severinov: Host RNA polymerase inhibitors encoded by ϕKMV-like phages of Pseudomonas. Virology 11/2012; 436(1)., DOI:10.1016/j.virol.2012.10.021

Olga Pavlova, Daria Lavysh, Evgeny Klimuk, Marko Djordjevic, Dmitry A Ravcheev, Mikhail S Gelfand, Konstantin Severinov, Natalja Akulenko: Temporal Regulation of Gene Expression of the Escherichia coli Bacteriophage phiEco32. Journal of Molecular Biology 02/2012; 416(3):389-99., DOI:10.1016/j.jmb.2012.01.002

Anastasia Graf, Maria Kabysheva, Eugeny Klimuk, Lidia Trofimova, Tatiana Dunaeva, Gregor Zündorf, Stefan Kahlert, Georg Reiser, Tatiana Storozhevykh, Vsevolod Pinelis, Natalia Sokolova, Victoria Bunik: Role of 2-oxoglutarate dehydrogenase in brain pathologies involving glutamate neurotoxicity. Journal of Molecular Catalysis B Enzymatic 11/2009; 61(1-2-61):80-87., DOI:10.1016/j.molcatb.2009.02.016

Victoria I Bunik, Maria S Kabysheva, Eugeny I Klimuk, Tatiana P Storozhevykh, Vsevolod G Pinelis: Phosphono Analogues of 2-Oxoglutarate Protect Cerebellar Granule Neurons upon Glutamate Excitotoxicity. Annals of the New York Academy of Sciences 09/2009; 1171(1):521-9., DOI:10.1111/j.1749-6632.2009.04709.x

L.K. Trofimova, A. V. Graf, E.I. Klimuk, M.S. Kabysheva, N. A. Sokolova, V.I. Bunik: P.1.d.011 Phosphonate analogue of ketoglutarate increases activity of ketoglutarate dehydrogenasecomplex in situ and in vivo. European Neuropsychopharmacology 08/2008; 18., DOI:10.1016/S0924-977X(08)70326-2

2003-2008 – student, Bioengineering and Bioinformatics School, Lomonosov Moscow State University (graduated with high honours )

2008-2013 – PhD student, Bioengineering and Bioinformatics School, Lomonosov Moscow State University (graduated with degree in molecular biology)

Research interests – Transcription regulation, transcription factors, inhibitors of RNA-polymerase, bacteriophages, DNA packaging motors, CRISPR, protein-protein interactions.

Projects supported with funding:

2012-2013 – Russian foundation for basic research grant № 12-04-31069 to study inhibitors of host RNA-polymerase within phages infecting M. tuberculosis

2014-2016 – grant from Dynasty Foundation to study mechanism of viral DNA packaging

2016-2018 – Russian foundation for basic research grant № 16-34-60137 to study regulation of R-M system Kpn2I

2016-2017 – Russian foundation for basic research grant (cooperative grant with Royal Society, UK) №16-54-10074 КО_а “Engineering thermophage particles for studying virus assembly”

2015-2017 – Russian Science Foundation grant 15-15-10017 “Investigation of new antibacterial agents: peptides, virulent bacteriophages and their cytotoxic proteins and lytic enzymes active against multidrug-resistant pathogens, design of novel molecular epidemiological typing schemes for monitoring bacterial pathogens”

2018-2020 – Russian Science Foundation grant 18-74-00123 to study DNA-protein interactions in terminal complexes of bacteriophages infecting thermophilic bacteria

 

2006 – Award of Academician S.E. Severin.

2011 – A winner of science & innovation contest for young researchers.

2014 – “Best poster presentation” award on “Mathematical Virology 2014” conference