alexeyyashchenok

Alexey Yashchenok

Leading Research Scientist
Center for Photonic Science and Engineering

Professor: Dmitry Gorin

PROFESSIONAL EXPERIENCE

11.2017 – present – Leading Research Scientist, Center for Photonics & Quantum Materials (CPQM), Skolkovo Institute of Science and Technology, Moscow, Russia

2017 – 11.2017 – Professor, Department of Nano- and Biomedical Technologies of Saratov State University, Saratov, Russia

09.2014 – 11.2017 – Group Leader, Research project “Remote controllable nanostructured systems for site-specific delivery and diagnostics”, Remote Controlled Theranostic Systems Lab, Institute of Nanostructures and Biosystem, Saratov State University, Russia

03.2014 – 08.2014 – Independent Researcher, Research project “Studies of cellular interaction by Raman spectroscopy”, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany

03.2012 – 02.2014 – Postdoctoral Fellow, Alexander von Humboldt Scholarship, Research project “Label-free single-molecule detection based on Raman scattering on self-assembled gold nanoparticles”, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany

07.2010 – 02.2012 – Postdoctoral Fellow, Research project “Nanometer and micrometer drug delivery vehicles for enzyme delivery”, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany

06.2009 – 06.2012 – Assistant Professor, Department of Nano- and Biomedical Technologies of Saratov State University, Saratov, Russia

07.2007 – 05.2009 – Head of School Laboratory, Department of Nano- and Biomedical Technologies of Saratov State University, Saratov, Russia

Short research Visits

09.2019 – 10.2019 Visiting Fellowship, Ghent University’s Russia Platform Short-term Fellowship Program, Department Molecular Biotechnology, Ghent University, Ghent, Belgium

02.2015 – 04.2015 – Research Visitor, cooperative research supported by Marie Curie Action – International Research Staff Exchange Scheme of European Union, Department Molecular Biotechnology, Ghent University, Ghent, Belgium

09.2009 – 11.2009 – Research Fellowship, DAAD scholarship (Referat 325 A/09/03626), Research project “Multicomponent nanocomposite microcontainers with remote control properties”, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany

02.2008 – 03.2008 – Research Visitor, EU Research project “Fabrication multifunctional microcontainers with remote controlling properties”, Queen Mary University of London School of Engineering and Material Sciences, London, United Kingdom

10.2007 – 12.2007 – Research Visitor, EU Research project “Fabrication multifunctional microcontainers with remote controlling properties”, Queen Mary University of London School of Engineering and Material Sciences, London, United, Kingdom

07.2007 – 09.2007 – Research Visitor, EU Research project “SELECTNANO”, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany

R&D of interrogated multifunctional micro and nanostructures both for in vitro and in vivo imaging, diagnostic and therapeutic applications.

The completion of the STRIP project (An Interface of Physics and Life Sciences for Cancer Diagnostics (short title: Platform for Detection of Human Health Markers at Point-of-Care)) led to the preparation of intellectual property and founding the spin-off company “TETRAQUANT” (July 26, 2019).

The most significant publications:

  1. Alexey M. Yashchenok, Vasiliy S. Chernyshev, Elena V. Konovalova, Roman Kholodenko, Ekaterina Tsydenzhapova, Victoria O. Shipunova, Alexey A. Schulga, Sergey M. Deyev, Dmitry A. Gorin Anti-CD63-Oligonucleotide Functionalized Magnetic Beads for the Rapid Isolation of Small Extracellular Vesicles and Detection of EpCAM and HER2 Membrane Receptors using DARPin Probes Analysis&Sensing, 2022, e202200059.
  2. T.O. Abakumova, O.I. Gusliakova, J. Cvjetinovic, O.I. Efimova, E.V. Konovalova, A.A. Schulga, T.S. Zatsepin, D.A. Gorin, A.M. Yashchenok, S.M. Deyev Barnase-Loaded Vaterite Nanoparticles Functionalized by EpCAM Targeting Vectors for the Treatment of Lung Diseases ACS Appl. Nano Mater. 2022, 5, 8, 10744-10754.
  3.  V.S. Chernyshev, R.N. Chuprov-Netochin, E. Tsydenzhapova, E.V. Svirshchevskaya, R.A. Poltavtseva, A. Merdalimova, A. Yashchenok, A. Keshelava, K. Sorokin, V. Keshelava, G.T. Sukhikh, D. Gorin, S. Leonov, M. Skliar Asymmetric depth-filtration: A versatile and scalable method for high-yield isolation of extracellular vesicles with low contamination J ExtracellVesicles. 2022, 11:e12256.
  4. Daniil Nozdriukhin, Sandeep Kumar Kalva, Weiye Li, Jim Zhao, Alexey Yashchenok, Dmitry Gorin, Daniel Razansky, and Xosé Luís Deán-Ben Optoacoustic visualization of individual core-shell microparticles in vivo, Proc. SPIE 11960, Photons Plus Ultrasound: Imaging and Sensing 2022, 1196016 (3 March 2022); https://doi.org/10.1117/12.2608617.
  5. Merdalimova, A.A.; Rudakovskaya, P.G.; Ermatov, T.I.; Smirnov, A.S.; Kosolobov, S.S.; Skibina, J.S.; Demina, P.A.; Khlebtsov, B.N.; Yashchenok, A.M.; Gorin, D.A. SERS Platform Based on Hollow-Core Microstructured Optical Fiber: Technology of UV-Mediated Gold Nanoparticle Growth. Biosensors 2022, 12, 19.
  6. Nozdriukhin, Daniil; Kalva, Sandeep; Li, Weiye; Yashchenok, Alexey; Gorin, Dmitry; Razansky, Daniel; Dean Ben, Xose Luis Rapid volumetric optoacoustic tracking of individual microparticles in vivo enabled by a NIR-absorbing gold-carbon shell ACS Applied Materials & Interfaces, 2021, 13, 41, 48423–48432.
  7. Elizaveta A. Maksimova, Roman A. Barmin, Polina G. Rudakovskaya, Olga A. Sindeeva, Ekaterina S. Prikhozhdenko, Alexey M. Yashchenok, Boris N. Khlebtsov, Gaoshan Huang, Yongfeng Mei, Krishna Kanti Dey, Alexander A. Solovev, Dmitry A. Gorin Bubble-micromotors Based on Albumin Functionalized with Gold Nanocages and Zinc Phthalocyanine for Multimodal Imaging Micromashines, 2021, 12, 1161. https://doi.org/10.3390/mi12101161.
  8. A. Yashchenok, O. Gusliakova, E. Konovalova, M. V. Novoselova, V. Shipunova, T. O. Abakumova, O. Efimova, R. Kholodenko, A. Shulga, T. Zatsepin, D. Gorin and S. Deyev, Barnase encapsulation into submicron porous CaCO3 particles – studies of loading and enzyme activity J. Mater. Chem. B, 2021, DOI: 10.1039/D1TB01315G.
  9. Vasiliy S. Chernyshev, Roman N. Chuprov-Netochin, Ekaterina Tsydenzhapova, Elena V. Svirshchevskaya, Rimma A. Poltavtseva, Anastasiia Merdalimova, Alexey Yashchenok, Amiran Keshelava, Konstantin Sorokin, Varlam Keshelava, Gennadiy T. Sukhikh, Dmitry Gorin, Sergey Leonov, Mikhail Skliar Asymmetric depth-filtration – a versatile and scalable approach for isolation and purification of extracellular vesicles bioRxiv preprint doi: https://doi.org/10.1101/2021.08.23.457333.
  10. D. Nozdriukhin, N. Besedina, V. Chernyshev, O. Efimova, P. Rudakovskaya, M. Novoselova, D. Bratashov, R. Chuprov-Netochin, R. Kamyshinsky, A. Vasiliev, D. Chermoshentsev, S.A. Dyakov, V. Zharov, N. Gippius, D.A. Gorin, A. Yashchenok Gold nanoparticle-carbon nanotube multilayers on silica microspheres: Optoacoustic-Raman enhancement and potential biomedical applications Materials Science & Engineering C 2021, 120, 111736.
  11. A.M. Yashchenok, V.S. Chernyshev, P.G. Rudakovskaya, A.A. Merdalimova, D.A. Gorin, V.O. Shipunova, A.A. Schulga, S.M. Deev “Nano- and microparticles for isolation of exosome subpopulations and their analysis” Russian Patent, conception of the technology: N 2733884, N 2020102930, Priority date 24/01/2020.
  12. Saveleva, E.S. Prikhozhdenko, D. Gorin, A.G Skirtach, A. Yashchenok, B. Parakhonskiy Polycaprolactone-based, porous CaCO3 and Ag nanoparticle modified scaffolds as a SERS platform with molecule-specific adsorption Frontiers in Chemistry Analytical Chemistry, 2020, doi: 10.3389/fchem.2019.00888.
  13. Cvjetinovic, A.I. Salimon, M.V. Novoselova, P.V. Sapozhnikov, E.A. Shirshin, A.M. Yashchenok, O.Yu. Kalinina, AM. Korsunsky, D.A. Gorin Photoacoustic and fluorescence lifetime imaging of diatoms Photoacoustics 18 (2020) 100171.
  14. A. Merdalimova, V. Chernyshev, D. Nozdriukhin, P. Rudakovskaya, D. Gorin, A. Yashchenok Identification and Analysis of Exosomes by Surface-Enhanced Raman Spectroscopy Appl. Sci., Special Issue “Surfaced Enhanced Raman Scattering (SERS) in Disease Diagnosis” 2019, 9(6), 1135; doi:10.3390/app9061135.
  15. R. Kamyshinsky, I. Marchenko, B. Parakhonskiy, A. Yashchenok, Y. Chesnokov, A. Mikhutkin, D. Gorin, A. Vasiliev, T. Bukreeva Composite materials based on Ag nanoparticles in situ synthesized on the vaterite porous matrices Nanotechnology, 2019, 30, 035603.
  16. E.S. Prikhozhdenko, D.N. Bratashov, V.S. Atkin, R.A. Kamyshinsky, A.L. Vasiliev, B.N. Khlebtsov, D.A. Gorin, H. Möhwald, A.M. Yashchenok Precise Control of Distance between Plasmonic SERS Substrate and Analyte Molecules with Polyelectrolyte Layers J Raman Spectrosc., 2018, 49,1581-1593.
  17. E.S. Prikhozhdenko, D.N. Bratashov, D.A. Gorin, A.M. Yashchenok Flexible SERS-active substrates made of nanofibrous membranes Nano Research, 2018, 11 (9), 4468–4488.
  18. А. Abalymov, R. Verhovskiy, M. Novoselova, B. Parakhonskiy, D. Gorin, A. Yashchenok, G. Sukhorukov Live-Cell Imaging by Confocal Raman and Fluorescence Microscopy Recognizes the Crystal Structure of Calcium Carbonate Particles in HeLa Cells Biotechnology Journal, 2018, DOI: 10.1002/biot.201800071.
  19. Ekaterina S. Prikhozhdenko, Daniil N. Bratashov, Andrei V. Sapelkin, Alexey M. Yashchenok, Gleb B. Sukhorukov, Goryacheva I.Yu. Solvothermal Synthesis of Hydrophobic Carbon Dots in Reversed Micelles Journal of Nanoparticle Research, 2018, DOI: 10.17632/cd4ht4dvp8.1.
  20. V. Parakhonskiy, A.M. Yashchenok, H. Mӧhwald, D. Volodkin, A.G. Skirtach Release from polyelectrolyte multilayer capsules in solution and on polymeric surfaces Advanced Materials Interfaces 2017, 4, 1600273.
  21. S. Prikhozhdenko, V.S. Atkin, B.V. Parakhonskiy, I.A. Rybkin, A. Lapanje, G.B. Sukhorukov, D.A. Gorin, A.M. Yashchenok New post-processing method of preparing nanofibrous SERS substrates with a high density of silver nanoparticles RSC Adv., 2016, 6, 84505–84511.
  22. Donatan, A. Yashchenok, N. Khan, B. Parakhonskiy, M. Cocquyt, B. Pinchasik, D. Khalenkow, H. Möhwald, M. Konrad, A. Skirtach, Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles, ACS Appl. Mater. Interfaces. 2016, 8 (22), 14284-14292.
  23. A.M. Yashchenok, J. Jose, P. Trochet, G.B. Sukhorukov, D.A. Gorin Multifunctional polyelectrolyte microcapsules as a contrast agent for photoacoustic imaging in blood J. Biophotonics 2016, 9, No. 8, 792-799.
  24. Parakhonskiy, M. V Zyuzin, A. Yashchenok, S. Carregal‑Romero, J. Rejman, H. Möhwald, W.J. Parak, A.G. Skirtach The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells J Nanobiotechnol 2015, 13, 53.
  25. Stetciura, I.Y., Yashchenok, A., Masic, A., Lyubin, E.V., Inozemtseva, O.A., Drozdova, M.G., Markvichova, E.A., Khlebtsov, B.N., Fedyanin, A.A., Sukhorukov, G.B., Gorin, D.A., Volodkin, D. Composite SERS-based satellites navigated by optical tweezers for single cell analysis Analyst 2015, 140, 4981-4986.
  26. A.N. Severyukhina, B.V. Parakhonskiy, E.S. Prikhozhdenko, D.A. Gorin, G.B. Sukhorukov, H. Moehwald, A.M. Yashchenok Nanoplasmonic Chitosan Nanofibers as Effective SERS Substrate for Detection of Small Molecules ACS Appl. Mater. Interfaces 2015, 7, 15466-15473.
  27. Yashchenok, A., Masic, A., Gorin, D., Inozemtseva, O., Shim, B.S., Kotov, N., Skirtach, A., Möhwald, H. Optical Heating and Temperature Determination of Core–Shell Gold Nanoparticles and Single-Walled Carbon Nanotube Microparticles. Small 2015, 11, 1320-1327.
  28. Parakhonskiy, B.V., Yashchenok, A.M., Donatan, S., Volodkin, D.V., Tessarolo, F., Antolini, R., Möhwald, H., Skirtach, A.G. Macromolecule loading into spherical, elliptical, star-like and cubic calcium carbonate carriers. ChemPhysChem. 2014, 15, 2817-2822.
  29. Parakhonskiy B.V., Yashchenok, A., Konrad, M., Skirtach, A.G. Colloidal Micro- and Nano- Particles as Templates for Polyelectrolyte Multilayer Capsules. Advances in Colloid and Interface Science 2014, 207, 253-264.
  30. Wuytens, B. Parakhonskiy, A. Yashchenok, M. Winterhalter, A. Skirtach Pharmacological aspects of release from microcapsules – from polymeric multilayers to lipid membranes Current Opinion in Pharmacology 2014, 18:129-140.
  31. Karamitros, C.S., Yashchenok, A.M., Möhwald, H., Skirtach, A.G., M. Konrad Preserving catalytic activity and enhancing biochemical stability of the therapeutic enzyme asparaginase by biocompatible multilayered polyelectrolyte microcapsules. Biomacromolecules 2013, 14, 4398-4406.
  32. Yashchenok, A., Parakhonskiy, B., Donatan, S., Kohler, D., Skirtach, A., Möhwald H. Polyelectrolyte multilayer microcapsules templated on spherical, elliptical and square calcium carbonate particles. J. Mater. Chem. B 2013, 1, 1223-1228.
  33. Yashchenok, A., Masic, A., Gorin, D., Shim, B.S., Kotov, N.A., Fratzl, P., Möhwald, H., Skirtach A. Nanoengineered Colloidal Probes for Raman-based Detection of Biomolecules inside Living Cells. Small 2013, 9, 351-356.
  34. Marchenko, I., Yashchenok, A. , Borodina, T., Bukreeva, T., Konrad, M., Möhwald, H., Skirtach, A. Controlled enzyme-catalyzed degradation of polymeric capsules templated on CaCO3: Influence of the number of LbL layers, conditions of degradation, and disassembly of multicompartments. Journal of Controlled Release 2012, 162, 599-605.
  35. Delcea, M., Sternberg, N., Yashchenok, A.M., Georgieva, R., Bäumler, H., Möhwald, H., Skirtach, A.G. Nanoplasmonics for Dual Molecule Release through Nanopores in the Membrane of Red Blood Cells. ACSNano 2012, 6, 4169-4180.
  36. Skirtach A.G., Yashchenok A.M., Möhwald H. Encapsulation, release and applications of LbL polyelectrolyte multilayer capsules. Chem. Commun. 2011, 47, 12736-12746.
  37. Yashchenok A.M., Delcea M., Videnova K., Jares-Erijman E.A., Jovin T.M., Konrad M., Möhwald H., Skirtach A.G. Enzyme Reaction in the Pores of CaCO3 Particles upon Ultrasound Disruption of Attached Substrate-Filled Liposomes. Angew. Chem. Int. Ed. 2010, 49, 8116-8120.
  38. Yashchenok, A.M., Bratashov, D.N., Gorin, D.A., Lomova, M.V., Pavlov, A.M., Sapelkin, A.V., Shim, B.S., Khomutov, G.B., Kotov, N.A., Sukhorukov, G.B., Möhwald, H., Skirtach, A.G. Carbon Nanotubes on Polymeric Microcapsules: Free-Standing Structures and Point-wise Laser Opening. Adv. Func. Mater. 2010, 20, 3136-3142.

Book contributions

E.S. Prikhozhdenko, D.A. Gorin, A.M. Yashchenok Raman spectroscopy, antiterrorism safety // Advanced technologies for aircraft industry: Analytical review – М.: «NAUKA», 2017 – 463 p.: images. ISBN 978-5-02-040100-6.Klimov B.N., Shtykov S.N., Gorin D.A., Inozemtceva O.A., Gluhovskoy E.G., Yashchenok A.M., Kolesnikova T.A. Physico-chemistry of nanostructured materials // Tutorial for students of nano- and biomedical technologies department of Saratov State University, Saratov, 2009, 218 p., ISBN 978-5-98116-055-4.

09/2014 – 01/2017         Doctor of Science in Biophysics, Saratov State University, Saratov, Russia

09/2004 – 05/2007          Candidate of Science in Physics, Saratov State University, Saratov, Russia,

09/2002 – 06/2004          Master of Science in Physics, Saratov State University, Saratov, Russia

09/1998 – 06/2002          Bachelor of Science in Physics, Saratov State University, Saratov, Russia

Design and fabrication of physically and chemically responsive micro- and nanostructures, drug delivery systems, nanobiotechnology, nanoplasmonics, label-free and single molecule detection. Biomedical applications of Raman and surface enhanced Raman microspectroscopy.

  • “A new approach for the treatment of viral infections of the respiratory tract, including SARS-CoV-2, based on targeted delivery of vaterite particles with encapsulated ribonuclease”, RFBR Foundation, Project 20-04-60150: (2020-2022, 6,000,000 per year), (Principal Investigator). Academic partners: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry.
  • “Bioactive ultrasound-driven microbubbles for theranostics” RFBR Foundation, Project  19-53-80047 БРИКC, (Executor).
  • “Platform for Detection of Human Health Markers at Point-of-Care (POC): An Interface of the Physical and Life Sciences”, the Skoltech Translational Research and Innovation Program, (Responsible Executor).
  • “Label-free single molecule detection based on Raman scattering on self-assembled gold nanoparticles”, Alexander von Humboldt Scholarship, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany (Principal Investigator).
  • “Multicomponent nanocomposite microcontainers with remote control properties”, German Academic Exchange Service (DAAD) Scholarship, (Referat 325 A/09/03626), Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany (Principal Investigator).
  • “SERS structures based on inorganic porous particles decorated with plasmon resonance nanoparticles as a sensor system for intracellular studies”, RFBR research project no. 15-29-01172 ofi_m (Responsible Executor).
  • “Fabrication and exploration of multifunctional microcontainers with remote controlling properties”, Joint Research Project Queen Mary University of London, United Kingdom – Saratov State University, Russian Federation, (Executor).
  • “Fabrication of water suspension of microcapsules with nanocomposite shells sensitive to electromagnetic irradiation”, Joint Research project between RFBR (Interaction of microwave irradiation with nanostructured materials № 06-02-04009) and DFG (436 RUS 113/844/0-1 Remote (microwave) activated release from composite nanoparticle/polymer microcapsules), (Executor).
  • “Fabrication of water suspension of microcapsules with nanocomposite shells sensitive to ultrasound”, Joint Research project between RFBR (Interaction of microwave irradiation with nanostructured materials № 06-02-04009) and DFG (436 RUS 113/844/0-1 Remote (microwave) activated release from composite nanoparticle/polymer microcapsules), (Executor).
  • “Fabrication of polyelectrolyte microcapsules with nanoparticles”, Government of the Russian Federation, No. 02.513.11.3043, “Functionalized nanoparticles with controlled plasmon resonance and polyelectrolyte microcapsules with nanoparticles in the shell”, 2007-3-1.3-07-01-081, (Executor).
  • “Fabrication and exploration of physical properties of nanocomposite micro- and nanostructures”, 2006-РИ-19.0/001/051, Government of the Russian Federation No. 02.442.11.7249, (Executor).
  • “Remote controlled nanostructured systems for targeted delivery and diagnostics”, Government of the Russian Federation (grant no. 14.Z50.31.0004 to support scientiаc research projects implemented under the supervision of leading scientists at Russian institutions and Russian institutions of higher education), (Executor).

08 – 10.2009                     German Academic Exchange Service (DAAD) Scholarship, Research project “Multicomponent nanocomposite microcontainers with remote control properties”, (Referat 325 A/09/03626), Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany

03.2012 – 03.2014           Alexander von Humboldt Scholarship, Research project “Label-free single molecule detection based on Raman scattering on self-assembled gold nanoparticles”, Max-Planck Institute of Colloids and Interfaces, Potsdam, Germany