konstantinseverinov

Download CV

Personal Websites

Konstantin Severinov

Professor, Director of the Center for Data-Intensive Biomedicine and Biotechnology
Center for Data-Intensive Biomedicine and Biotechnology

Konstantin is a professor at the Molecular Biology and Biochemistry department at Rutgers University, New Brunswick, and a head of laboratory at the Waksman Institute for Microbiology. He also heads a Laboratory of genetic regulation of prokaryotic mobile genetic elements at the Institute of Molecular Genetics at the Russian Academy of Sciences and a Laboratory of molecular genetics of microorganisms at the Institute of Gene Biology at the Russian Academy of Sciences.

He is a member of the Science and Technology Council of the Russian Nanotechnology corporation and a consultant for 7 biotech companies and several Russian government agencies. He has produced over 200 peer-reviewed publications and 6 patents.

Konstantin works on genetic, biochemical, and structural analyses of transcription mechanism and regulation in bacteria. He also studies the regulation of macromolecular synthesis during the process of bacteriophage infection and various molecular interactions between bacteriophages and their host bacteria. Another main direction of his research involves analysis of antibacterial peptides called microcins: their structures, mechanisms of function, evolution, and the use as new antibiotics.

  • RNA transcription
  • Bacterial RNA polymerase structure-function and mechanism
  • Bacteriophage development and interactions with bacterial hosts
  • Structure-activity analysis of antibacterial peptides
  • Studies of bacterial diversity in extreme environments
  • Zenkin, N., Naryshkina, T., Kuznedelov, K., and Severinov, K. (2006) The molecular mechanism of replication primer synthesis by RNA polymerase. Nature, 439, 617-620
  • Zenkin, N., Yuzenkova, Y., and Severinov, K. (2006) Transcriptional proofreading through a ribozyme-like activity of the nascent transcript. Science, 313, 518-520
  • Zenkin, N., Kulbachinskiy, A., Yuzenkova, Y., Mustaev, Bass, I., Severinov, K., and Brodolin, K. (2007) Region 1.2 of the RNA polymerase σ subunit modulates recognition of the -10 promoter element. EMBO J., 26, 955-964
  • Pavlova, O., Mukhopadhyay, J., Sineva, E., Ebright, R. H., and Severinov, K. (2008) Systematic structure-activity analysis of microcin J25. J. Biol. Chem., 283, 25589-25595
  • Van de Vijver, P., Vondenhoff, G. H. M., Kazakov, T., Semenova, E., Kuznedelov, K., Metlitskaya, A., Van Aerschot, A., and Severinov, K. (2009) Synthetic Microcin C analogs targeting different aminoacyl-tRNA synthetases. J. Bacteriol., 191, 6273-6280
  • Pougach, K., Semenova, E., Bogdanova, E., Datsenko, K.A., Djordjevic, M., Wanner, B. L., and Severinov, K. (2010) Transcription, transcript processing and function of E. coli CRISPR locus. Mol. Microbiol., 77, 1367-1379
  • Mekler, V., Pavlova, O., and Severinov, K. (2011) The interaction of E. coli RNA polymerase σ70 subunit with promoter elements in the context of free σ70, RNA polymerase holoenzyme, and the β’-σ70 complex. J. Biol. Chem., 286, 270-279
  • Semenova, E., Jore, M. M., Datsenko, K. A., Semenova, A., Westra, E. R., Wanner, B. L., van der Oost, J., Brouns, S. J. J., and Severinov, K. (2011) A crRNA seed sequence governs CRISPR interference. Proc. Natl. Acad. Sci. USA, 108, 10098-10103
  • Agarwal, V., Tikhonov, A., Metlytskaya, A., Severinov, K., and Nair, S. (2012) Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis inhibitor Microcin C7. Proc. Natl. Acad. Sci. USA, 109, 4425-30
  • Datsenko, K. A., Pougach, K., Tikhonov, A., Wanner, B. L., Severinov, K., and Semenova, E. (2012) Prior encounters dramatically stimulate adaptive bacterial CRISPR immune response to viruses. Nature Commun., 3, 945
  • Fulbright Senior Scholar (2010)
  • Fellow of American Academy of Microbiology (2009)
  • The Russian Science Support Foundation Prize (2008, 2009)
  • Doctor of Sciences, Russian Academy of Sciences, cum laude, (2006)
  • 10th Johnson & Johnson Discovery Award (1997)
  • The Burroughs Welcome Fund Career Award in Biomedical Sciences (1996-2002)
ekaterinasavitskaya
Ekaterina Savitskaya
Senior Research Scientist
dmitryghilarov
Dmitry Ghilarov
Research Scientist
evgenyklimuk
Evgeny Klimuk
Research Scientist
vasilysitnik
Vasily Sitnik
Research Scientist
Basic Molecular Biology Techniques
Number of ECTS credits: 3
Course Classification: Science, Technology, and Engineering

Course description:

The course provides hand-on experience with the general techniques used in the molecular biology/biotechnology lab. The goal of the course is to teach students good laboratory practice and rational planning of the experimental work.
There are three different levels to the course (basic, advanced and research).

Prerequisites:
The course requires basic knowledge of molecular and cellular biology.

Advanced Molecular Biology Techniques
Number of ECTS credits: 3
Course Classification: Science, Technology, and Engineering

Course description:
The course provides experience with the scientific project planning and implementation in the molecular biology/biotechnology lab.The goal of the course is to teach students good laboratory practice and rational planning of the experimental work.

Prerequisites:
Basic knowledge of standard molecular biology techniques (PCR, electrophoresis, cloning, protein expression/purification).

Advanced Molecular Biology Techniques 2 (research)

Number of ECTS credits: var
Course Classification: Science, Technology, and Engineering

Course description:
This project-based course provides experience with scientific project planning and implementation in the molecular biology/biotechnology lab. Course-based research projects are geared towards the background and experience of students.
The goal of the course is to teach students good laboratory practice and rational planning of the experimental work.

Prerequisites:
Basic knowledge of molecular and cellular biology as well as minimal wet lab experience.

Molecular Biology Seminar

Number of ECTS credits: 2
Course Classification: Science, Technology, and Engineering

Course description:
A research paper-based course where recent papers in the field are presented by students and critically discussed by the class led by instructor.

Prerequisites:
General Molecular Biology course.

1. “How can we change availability of the modern medicine genomic analysis?”, 17/11/2014

2. “Gene transcription”, 05/11/2014

3. “Protein folding”, 17/10/2014

4. “Genetic variations in bacteria”, 14/10/2014

5. Talk about the principles of sugar substitutes, experiments with laboratory mice and human microbiota, 13/10/2014

6. “Transfer of the biological signals”, 09/10/2014

7. “How does the mammal’s intestinal microflora protection mechanism works?”, 03/10/2014

8. “Macromolecules interactions”, 01/09/2014

9. Talk about the problems of biology teaching at school, 20/08/2014

10. “Systems biology and antibiotics crisis”, 04/07/2014

11. “Science sexism”, 04/06/2014

12. “Structural and functional regulation features of the bacterial transcription”, 17/05/2014

13. “How does the DNA with synthetic nucleotides has been built into a living cell?”, 13/05/2014

14. Talk about the scientific infrastructure, relationship between viruses and bacteria, and the main motivation of scientists, 30/04/2014

15.  “Why do we need bioinformatics?”, 23/12/2013

16.  “Genetic noise”, 26/11/2013

17. “Coevolution of humans and bacteria”, 15/11/2013

18. “Synthetic microbiology” 06/11/2013

19. “Gene therapy”, 03/09/2013

20. Talk about the photosynthesis process, oxidant problems, oxygen storage and transport proteins, 06/07/2013

21. Talk about the future of RAS, 02/07/2013

22. “Recommendations: 5 books about genetics”, 29/06/2013

23. “Microbiota of the human”, 08/05/2013

24. “Mitochondrial Eve”, 24/04/2013

25. Talk about the hemoglobin, 04/03/2014

ФИО: Северинов Константин Викторович

Занимаемая должность (должности): Директор ЦНИО

Преподаваемые дисциплины: Лабораторный курс “Базовые методы в молекулярной биологии”

Ученая степень: Доктор биологических наук, 2007; Кандидат биологических наук, 1993 Институт молекулярной генетики РАН Москва

Ученое звание (при наличии): Профессор по специальности “Молекулярная генетика”

Наименование направления подготовки и/или специальности: Биохимия

Данные о повышении квалификации и/или профессиональной переподготовке (при наличии): нет

Общий стаж работы: 25 лет

Стаж работы по специальности: 25 лет